Splice site selection by intron al3 of the C0X1 gene from Saccharomyces cerevisiae

Interactions of the 5 ' and 3 ' splice sites with intron internal sequences of the yeast mitochondrial group I intron al3 were studied using mutation analysis. The results can be fully explained by the splice guide model in which the splice sites are defined by the Internal Guide Sequence. No evidence was found for an alternative interaction between intron nucleotides preceding the 3 ' splice site and nucleotides in the vicinity of the core region as was found for the Tetrahymena Intron. Our results also suggest that binding of the 5 ' and 3 ' splice site nucleotides to the IGS can not take place simultaneously. The intron must therefore undergo conformatlonal changes as the reaction proceeds from the first step of self splicing, GTP attack at the 5 ' splice site, to exon ligation, the second step