VIROLOGY 113,769-'7'75 (1981) Physical Map of the Bacteriophage P22 Genome

P22 mutations have been located relative to restriction endonuclease cleavage sites on the P22 chromosome by rescue of genetic markers from cloned DNA fragments. These data, in combination with prior genetic and physical mapping data, have allowed the construction of a detailed composite physical and genetic map. Many cleavage sites for restriction en-donucleases have now been mapped on the chromosome of bacteriophage P22 (1-5). This physical map of cleavage sites facil-itates generation of defined genome seg-ments for a variety of applications. Many such applications require information about the location of genes relative to re-striction sites. In previous work, we have oriented the genetic map of P22 with the restriction endonuclease cleavage site ma