In vitro antibacterial activities of root-canal sealers by using two different methods

The purpose of this in vitro study was to evaluate the antibacterial activity of five different root-canal sealers (RoekoSeal, Ketac-Endo, AH Plus, Sealapex, Sultan). With the use of Enterococcus faecalis as a test organ-ism, both the agar-diffusion test (ADT) and direct-con-tact test (DCT) were performed. For DCT, sealers were mixed and placed on the sidewall of microtiter plate wells. A 10-l bacterial suspension was placed on the tested material samples. Bacteria were allowed to di-rectly contact to the sealers for 1 h at 37°C. Bacterial growth was then spectrophotometrically measured through every 30 min for 19 h by using an Anthos Labtec HT 2. For ADT, a 200-l bacterial suspension was spread on brain-heart infusion agar plates. Freshly mixed sealers were poured into uniform wells punche