Immunohistochemical study of steroidogenesis and cell proliferation in polycystic ovarian syndrome. Hum Reprod 11

• ̂ To whom correspondence should be addressed We evaluated the immunolocalization of the steroidogenic enzymes involved in the production of ovarian steroids, including the cholesterol side-chain cleavage enzyme (P450scc), 3p"-hydroxysteroid dehydrogenase (HSD), 17a-hydroxylase (P450cl7) and aromatase (P450arom), oestro-gen receptor (ER) and androgen receptor (AR), a steroido-genic transcription factor, Ad4-binding protein (Ad4BP) and a cell cycle-related nuclear antigen, Ki67, in five patients with polycystic ovarian syndrome (PCOS). Results were compared with those from normal cycling human ovaries to study in situ ovarian steroidogenesis and cell proliferation in polycystic ovaries (PCO). We classified the follicles morphologically according to the development of granulosa cells: type A, more than four layers (n = 7)