DNA replication and UV-induced DNA repair synthesis in human fibroblasts are much less sensitive than DNA polymerase alpha to inhibition by butylphenyl-deoxyguanosmie triphosphate

In mammalian cells, both semiconservative DNA replication and the DNA repair patch synthesis Induced by high doses of ultraviolet radiation are known to be Inhibited by aph1d1col1n, Indicating the Involvement 1n these processes of one or both of the aph1d1col1n-sensit1ve DNA polymerases, a and/or 4. In this paper, N2-(p_-n-butylphenyl)-2 '-deoxyguanosine-5'-triphosphate, a strong Inhibitor of polymerase a and a weak Inhibitor of polymerase 4, 1s used to further characterize the DNA poiymerase(s) Involved 1n these two forms of nuclear DNA synthesis. In permeable human fibroblasts, DNA replication and ultraviolet-Induced DNA repair synthesis are more resistant to the Inhibitor than DNA polymerase a by factors of approximately 500 and 3000, respectively. These findings are most consistent with the Involvement of DNA polymerase 4 1n these processes