Copyright) 1992, American Society for Microbiology Isolation of Vibriostatic Agent 0/129-Resistant Vibrio cholerae Non-O1 from a Patient with Gastroenteritis

A strain of Vibrio cholerae non-Ol was isolated from a 48-year-old male in California who presented with a chief complaint of watery diarrhea. Laboratory investigations of this strain revealed the bacterium to be resistant to trimethoprim-sulfamethoxazole and to the vibriostatic agent 0/129 (2,4-diamino-6,7-diisopropyl-pteridine). The family Vibrionaceae is composed of oxidase-positive, facultatively anaerobic bacilli that utilize D-glucose as a source of energy and are motile by means of polar flagella (4). Three genera (Vibrio, Aeromonas, and Plesiomonas) in this family are recognized human pathogens causing a variety of gastrointestinal or extraintestinal diseases. The initial generic identification of members of this group in the clinical laboratory is based upon a small set of dif-ferential morphologic and biochemical criteria that in-clude growth on TCBS (thiosulfate-citrate-bile salts) agar and susceptibility to the vibriostatic agent 0/129 (2,4-di