Correlation Between Myeloperoxidase- Quantified Neutrophil Accumulation and Ischemic Brain Injury in the Rat Effects of Neutrophil Depletion

in the pathogenesis of ischemia-reperfusion injury. The aim of the present study was to evaluate the correlation between neutrophil infiltration into ischemic tissues and brain injury after transient focal ischemia. Methods We evaluated the effects of depletion of circulat-ing neutrophils by administration of an antineutroph.il mono-clonal antibody (RP3) on brain edema formation, infarct size, and neutrophil infiltration (myeloperoxidase [MPO]-quanti-fied) in rats with 1 hour of middle cerebral artery (MCA) occlusion. Results In the cerebral cortex perfused by the anterior cerebral artery (ACA area), there was a significant increase in MPO activity only 24 hours (P<.05) after reperfusion. In the cerebral cortex perfused by the middle cerebral artery (MCA area) and caudate putamen, MPO activity was significantl