Additional platelet membrane glycoprotein abnormalities in Glanzmann's thrombasthenia: A comparison with normals by high resolution two-dimensional polyacrylamide gel electrophoresis

Bernard-Soulier syndrome have been analyzed by sur-face-labeling coupled with two-dimensional gel elec-trophoresis and compared with normals. As well as the previously described absence or deficiency in glyco-protein (GP) Ib(a) it could be shown that GP Ib,3 and an additional low molecular weight glycoprotein GP75.8-65 were not detectable using carbohydrate-la-beling methods or deficient to the same extent as the GPIba subunit. In addition, the thrombin cleavable glycoprotein could not be detected using carbohy-drate-labeling methods in two patients and was defi-cient in a third. This finding was confirmed in a fourth patient by one-dimensional gel electrophoresis. Thus, the changes in the membrane of Bernard-Soulier plate-lets are more complex than previously thought