A 26-mer DNA probe was designed from N-terminal sequence data for the cephalosporin 7a-hydroxylase (CH) of Streptomyces clavuligerus NRRL 3585 and used to screen a DNA library from this organism. The library was constructed in the AGEM-1l phage system. After plaque purification and reprobing, positive recombinant phages were chosen for further analysis. Characterization of the cloned DNA by restriction mapping and Southern hybridization showed that a 1.5-kb SailI fragment hybridized to the probe. Polymerase chain reaction assays using this fragment as a template and the probe as a primer indicated that the fragment carries the entire putative CH gene (cmcl). This was confirmed through the expression ofCH enzymatic activity when the fragment...
Genomic libraries of S. lavendulae and S. subrutilus were constructed in S. lividans using the tech...
A gene (bla) encoding a beta-lactamase is present in the cephamycin gene cluster of Streptomyces cla...
The isolation, sequencing, and study of the bacteriophages which target this genus, may yield invalu...
Streptomyces clavuligerus is a filamentous bacterium renowned for its production of medicinally rele...
The threat to public health posed by drug-resistant bacteria is rapidly increasing, as some of healt...
Streptomyces hygroscopicus 10-22 could not be transformed with any of the commonly used Streptomyces...
By using the promoter-probe plasmid pIJ424, genomic DNA fragments of Actinomadura R39 were shown to ...
The choice of an expression system for the metagenomic DNA of interest is of vital importance for th...
Streptomyces rochei A2 endoglucanase (eglS) and β-glucosidase (bgs1) genes were used as probes to su...
Streptomyces rochei A2 endoglucanase (eglS) and β-glucosidase (bgs1) genes were used as probes to su...
Wehave developed a method for gene replacement in Streptomyces hygroscopicus which permits introduct...
Streptomyces rochei A2 endoglucanase (eglS) and β-glucosidase (bgs1) genes were used as probes to su...
A 19 kb SphI DNA fragment containing the gene for the extracellular active-site serine beta-lactamas...
Phenoxazinone synthase (PHS) catalyzes the penultimate step in the actinomycin D biosynthetic pathwa...
A method for fractionating genomic DNA from Streptomyces spp. 5A DNA into 6.6 to 23 kilobase (kb) fr...
Genomic libraries of S. lavendulae and S. subrutilus were constructed in S. lividans using the tech...
A gene (bla) encoding a beta-lactamase is present in the cephamycin gene cluster of Streptomyces cla...
The isolation, sequencing, and study of the bacteriophages which target this genus, may yield invalu...
Streptomyces clavuligerus is a filamentous bacterium renowned for its production of medicinally rele...
The threat to public health posed by drug-resistant bacteria is rapidly increasing, as some of healt...
Streptomyces hygroscopicus 10-22 could not be transformed with any of the commonly used Streptomyces...
By using the promoter-probe plasmid pIJ424, genomic DNA fragments of Actinomadura R39 were shown to ...
The choice of an expression system for the metagenomic DNA of interest is of vital importance for th...
Streptomyces rochei A2 endoglucanase (eglS) and β-glucosidase (bgs1) genes were used as probes to su...
Streptomyces rochei A2 endoglucanase (eglS) and β-glucosidase (bgs1) genes were used as probes to su...
Wehave developed a method for gene replacement in Streptomyces hygroscopicus which permits introduct...
Streptomyces rochei A2 endoglucanase (eglS) and β-glucosidase (bgs1) genes were used as probes to su...
A 19 kb SphI DNA fragment containing the gene for the extracellular active-site serine beta-lactamas...
Phenoxazinone synthase (PHS) catalyzes the penultimate step in the actinomycin D biosynthetic pathwa...
A method for fractionating genomic DNA from Streptomyces spp. 5A DNA into 6.6 to 23 kilobase (kb) fr...
Genomic libraries of S. lavendulae and S. subrutilus were constructed in S. lividans using the tech...
A gene (bla) encoding a beta-lactamase is present in the cephamycin gene cluster of Streptomyces cla...
The isolation, sequencing, and study of the bacteriophages which target this genus, may yield invalu...