Developmentand Evaluationof a Methodfor Quantitationof Plasma High-Density-LipoproteinCholesterol

An improved method for separating hii-density lipoprotein with use of “Alphachol ” tubes containing pre-dispensed and lyophilized heparin and MnCl2 is described. Results for high-density lipoproteincholesterol(C-HDL) isolated by ultracentrifugation from a 360 mg/liter and a 510 mg/liter pool were compared with those for the same pools by the Alphachol (358 and 506 mg/liter) and standard Lipid Research Clinic (LRC) (354 and 504 mg/liter) procedures. The within-day coefficient of variation (CV) for the Al-phachol method was 1%, as compared to 1.3 % for the standard LRC method. The CV for between-day precision studiesconducted over 20 days was 5.3 % forthe Alpha-chol,4.2 % forthe standardLRC procedures.Analysisof C-HDL in 100 patients by both the Alphachol and the standard LRC method showed mean C-HDL valuesto b