The pancreatic-adrenocortical-pituitary clamp technique for study of counterregulation in humans

creatic-adrenocortical-pituitary clamp technique for study of counterregulation in humans. Am. J. Physiol. 252 (Endocrinol. Metab. 15): E565-E570, 1987.-The present experiments were undertaken to develop an approach to analyze the contribution of individual glucose counterregulatory hormones in humans. For this purpose, 24 normal subjects were studied twice: once (control experiments) hypoglycemia was induced by subcuta-neous infusion of insulin; and once [pancreatic-adrenocortical-pituitary (PAP) clamp technique] the spontaneous responses of plasma glucagon, growth hormone, and cortisol to hypogly-cemia were prevented by intravenous somatostatin and oral metyrapone, respectively, and each hormone was infused at variable rates, which reproduced spontaneous changes in their circulating concentrations in the control experiments. Plasma glucose rate of decrease (0.052 t 0.003 vs. 0.06 k 0.003 mg