Radiation-inducible promoter mediated PUMA gene in the the treatment of Tca8113

Objective: The aim of this study is to explore the therapeutic effect of the PUMA gene mediated by ra-diation-inducible promoters in the treatment of ton-gue squamous cell carcinoma. Methods: Recombi-nant pcDNA3.1 (+)/E-PUMA was constructed, in which the PUMA gene was mediated by a synthetic radiation inducible promoter. The recombined plas-mids were transfected into the Tca8113 cell and xeno-grafts of human tongue squamous carcinoma in na-ked mice respectively. After 24 h, the tumors were tr-eated with 3 Gy of irradiation to upregulate the PU-MA gene expression. PUMA mRNA was detected by RT-PCR. Proliferating cell nuclear antigen (PCNA) and apoptosis were detected by immunohistochemi