SUMMARY

Protoplasts isolated from Nicotiana tabacum (cv. Xanthi) leaves were infected with cucumber green mottle mosaic virus (CGMMV). Progeny CGMMV particles were first detected in the protoplasts at 24 h after inoculation. They were in small crystalline arrays confined in the ground cytoplasm. The earliest detectable cytological modification in the protoplasts was the presence of peripheral vesicles in the mitochondria t 24 h after inoculation. No other effects on the cytoplasm or organelles were noted