Plasma Lipase Properties as Related to Pancreatic Condition

We examined the sensitivity to colipase of two types of lipase (EC 3.1.1.3) activity in plasma. Results were very similar for plasma lipase corresponding to that found in cases of acute pancreatitis and for swine pancreas lipase, whereas we found some differences between “pancreatitis lipase ” and lipase from healthy subjects. Gel-filtration experiments sug-gest that the two forms of Iipase in plasma have different relative molecular masses; moreover, their avidity for anti-bodies against human pancreatic lipase differs. Guided by these studies, we propose optimal conditions for nephelome-try of “pancreatitis ” lipase. Additional Keyphrases: multiple forms pancreatitis. pase- monitoring therapy. deoxycholate. nephelometiy Determination of lipase (EC 3.1.1.3) activity in serum or plasma, as is the case for amylase activity, is now wef