J. Biochem. 99, 477^fS3 (1986) Bile Alcohol Profiles in Bile, Urine, and Feces of a Patient

Bile alcohols in bile, urine, and feces of a patient with cerebrotendinous xantho-matosis have been analyzed by a combination of capillary gas-liquid chromatography and mass spectrometry after fractionation into groups according to mode of con-jugation. The presence of at least 18 bile alcohols, which were excreted mainly as glucurono-conjugates in bile and urine, and as unconjugated forms in feces, was demonstrated. The following bile alcohols were identified with certainty by direct comparison with reference compounds: 5/S-cholestane-3a,7a,12a-triol; (23R)-5/3-cholestane-3a,7a,12a,23-tetrol; 5a- and 5/?-cholestane-3a,7a,12a,24-tetrols; 5a- an