Carrier-bound Methotrexate. III. ‡ Antiproliferative Activity of Macromolecular MTX Conjugates Against the Human HeLa and Colo Carcinoma

In continuation of studies in these laboratories aiming at the bioevaluation of macromolecular anticancer drug models, in vitro cytotoxicity screens are performed on several series of water-soluble polymer-methotrexate conjugates. The methotrexate drug in these conjugates is bound through amide or ester linkages to water-soluble polyamide- or polyamidoamine-type carriers by previously developed anchoring techniques. Tests were conducted against the HeLa human cervical carcinoma cell line generally considered to be drug-sensitive, and against two variants of the rather refractory Colo 320 DM, a human colon adenocarcinoma line