Add to ORKGThe epidermis expresses cyclooxygenases, lipoxygenases, and cytochromes P450, which utilize arachidonic acid to gen-erate a diverse array of lipid mediators affecting epidermal cellular differentiation and functions. Recent studies show that mouse epidermis expresses CYP2B19, a keratinocyte-specific epoxygenase that generates 11,12- and 14,15-epoxyeicosa-trienoic (EET) acids from arachidonate. We studied CYP2B19-dependent metabolism in mouse epidermal microsomes, re-constituted in the presence of [1-14C]arachidonic acid. The majority of the 14C products formed independently of NADPH, indicative of robust epidermal cyclooxygenase and lipoxygen-ase activities. We studied two NADPH-dependent products generated in a highly reproducible manner f...
Leukotriene B4 (LTB4, 5-12-dihydroxy 6,8,10,14-eicosatetraenoic acid), an enzyme-catalyzed oxidation...
Incubations of [14C]arachidonic acid ([14C]AA) with cell-free preparations from normal, clinically i...
CYP2C enzymes epoxidize arachidonic acid (AA) to metabolites involved in the regulation of vascular ...
This article has not been copyedited and formatted. The final version may differ from this v rsion
The established mouse epidermis-derived cell line HEL/30 was incubated in the presence of 3H arachid...
In these studies, we examined the possibility that cell-free preparations from murine keratinocytes ...
The levels of free arachidonic acid and 12L-hydroxy-5,8,10,14-eicosatetraenoic acid (12-HETE) are el...
This study was conducted to investigate the metabolism of arachidonic acid in the skin of hairless m...
Synthesis of 12- and/or 15-HETE by human epidermal cells was investigated after separating basal cel...
Epidermal cells were isolated from adult inbred SENCAR (SSIN) mice and separated by density-gradient...
The present studies demonstrate that incubation of arachidonic acid (AA) with a 20,000g homogenate (...
Neonatal mice deficient in IκB-α, an inhibitor of the ubiquitous transcription factor NF-κB, develop...
The production and metabolism of lipoxygenase eicosanoids were studied in cultured human keratinocyt...
Keratinocytes have recently been recognized as a source of mediators of cellular immune function. We...
Studies of drug and carcinogen metabolism in cultured keratin-ocytes and in intact epidermis from th...
Leukotriene B4 (LTB4, 5-12-dihydroxy 6,8,10,14-eicosatetraenoic acid), an enzyme-catalyzed oxidation...
Incubations of [14C]arachidonic acid ([14C]AA) with cell-free preparations from normal, clinically i...
CYP2C enzymes epoxidize arachidonic acid (AA) to metabolites involved in the regulation of vascular ...
This article has not been copyedited and formatted. The final version may differ from this v rsion
The established mouse epidermis-derived cell line HEL/30 was incubated in the presence of 3H arachid...
In these studies, we examined the possibility that cell-free preparations from murine keratinocytes ...
The levels of free arachidonic acid and 12L-hydroxy-5,8,10,14-eicosatetraenoic acid (12-HETE) are el...
This study was conducted to investigate the metabolism of arachidonic acid in the skin of hairless m...
Synthesis of 12- and/or 15-HETE by human epidermal cells was investigated after separating basal cel...
Epidermal cells were isolated from adult inbred SENCAR (SSIN) mice and separated by density-gradient...
The present studies demonstrate that incubation of arachidonic acid (AA) with a 20,000g homogenate (...
Neonatal mice deficient in IκB-α, an inhibitor of the ubiquitous transcription factor NF-κB, develop...
The production and metabolism of lipoxygenase eicosanoids were studied in cultured human keratinocyt...
Keratinocytes have recently been recognized as a source of mediators of cellular immune function. We...
Studies of drug and carcinogen metabolism in cultured keratin-ocytes and in intact epidermis from th...
Leukotriene B4 (LTB4, 5-12-dihydroxy 6,8,10,14-eicosatetraenoic acid), an enzyme-catalyzed oxidation...
Incubations of [14C]arachidonic acid ([14C]AA) with cell-free preparations from normal, clinically i...
CYP2C enzymes epoxidize arachidonic acid (AA) to metabolites involved in the regulation of vascular ...