Casein Kinase 2 Associates with the Yeast Chromatin Reassembly Factor Spt2/Sin1 To Regulate Its Function in the Repression of Spurious Transcription

Spt2/Sin1 is a DNA binding protein with HMG-like domains. It plays a role in chromatin modulations associated with transcrip-tion elongation in Saccharomyces cerevisiae. Spt2 maintains the nucleosome level in coding regions and is important for the in-hibition of spurious transcription in yeast. In this work, we undertook a biochemical approach to identify Spt2-interacting part-ners. Interestingly, casein kinase 2 (CK2) interacts with Spt2 and phosphorylates it in vitro as well as in vivo on two smal