Development of electrochemically gene-analyzing method using DNA-modified electrodes

For electrochemical DNA sequencing, several redox-responsive uridines modified at C5 were synthesized and site-specifically incorporated into DNA. Self-assembled DNA monolayers were prepared on a gold electrode using the dsDNA containing modified uridines. By measuring differential pulse voltammetry (DPV) using DNA-modified electrodes, the electro-chemical response was strongly affected by the site of the redox-responsive uridine in dsDNA