ca2' role in myogenic and neurogenic activities of canine ileum circular muscle

neurogenic activities of canine ileum circular muscle. Am. J. Physiol. 271 (Gastrointest. Liver PhysioZ. 34): G1053-G1066, 1996.-The role of Ca2+ in myogenic and neural activation in canine ileum circular muscle (CM) was studied during simul-taneous recordings of contractile and electrical activity in cross-sectioned slabs of muscularis externa or of isolated CM with deep muscular plexus (DMP) intact. Ca2+-free Krebs solution abolished inhibitory junction potentials (IJP) and contractions before changes in CM membrane potentials and while slow waves (SW) persisted at lower amplitude and frequency. This medium abolished SW more rapidly in iso-lated CM than in intact muscle strips and affected triggered SW (TSW) by lOO-ms pulses recorded near myenteric plexus or near DMP differentially in the full-thickness preparation; TSW did not occur in isolated CM. Ni2+, a nonselective Ca2+ channel antagonist, left IJP unchanged and reduced contrac-tions, frequencies, and amplitudes of spontaneous SW and TSW, but increased their durations. Nifedipine abolished contractions but SW, TSW, and IJP were unaffected. Cyclopia-zonic acid (CPA) increased SW frequency, produced spikes on SW plateaus, and increased CM tone, but did not affect IJP or resting membrane potentials. In nifedipine-pretreated strips, CPA decreased SW frequencies and amplitudes, evoked less tone, depolarized membrane potentials, and left IJP unal-tered. The neuronal N-type Ca 2+ channel blocker m-conotoxin GVIA abolished IJP, without affecting SW or TSW. We conclude that Ca2+ influx, not through L- or N-type Ca2+ channels, helps initiate ileal SW; L-type Ca2+ channels provide Ca 2+ for contraction and N-type Ca2+ channels pro-vide Ca2+ for IJP mediator release. Frequencies of SW may be modulated by uptake of Ca2+ into pacemaker stores. configurations in various regions of the canine gut (2,3