1 Novel Stage Classification of Human Spermatogenesis Based on Acrosome

To date, in the human seminiferous epithelium only 6 associations of cell types have been distinguished, subdividing the epithelial cycle into 6 stages of very different duration. This hampers comparisons between studies on human and laboratory animals in which the cycle is usually subdivided into 12 stages. We now propose a new stage classification on basis of acrosomal development made visible by immunohistochemistry (IHC) for (pro)acrosin. IHC for acrosin gives results that are comparable to periodic acid Schiff (PAS) staining. In the human too, we now distinguish 12 stages that differ from each other in duration by a factor of two, at most. B spermatogonia are first apparent in stage I, preleptotene spermatocytes are formed in stage V, leptonema starts in stage VII and spermiation takes place at the end of stage VI. A similar timing was previously observed in several monkeys. Stage identification by way of IHC for acrosin appeared possible for tissue fixed in formalin, Bouin fixative, diluted Bouin fixative, Cleland fluid and in modified Davidson fixative, indicating a wide applicability. In addition, it is also possible to distinguish the 12 stages i