Structure of the galactokinase gene of Escherichia coti, the last (?) gene of the gal operon C. Debouck * +, A.Riccio1*, D.Schumperli2*, K.McKenney3*, J.Jeffers4+, C.Hughes5+ and

We present the nucleotide sequence of the galactokinase gene (galK) of Escherichia coll Including Its 5 ' and 3 ' flanking regions. This DNA sequence derives from the Agal8 transducing phage and is identical to the sequence present in the galK gene fusion vectors, pKO and pKG, commonly used to study transcrlptional regulatory elements. We define the precise 3 ' junction between the bacterial and phage sequences in Xgal8 and demonstrate that this junction probably results from a homologous recombination event between identical 9 bp sequences common to the gal operon and phage X. Moreover, we examine the 300 bp region located immediately beyond galK for transcription termination function and find no gal operon terminator. Lastly, we compare the galK genes of E_. coll and the yeast S_. cerevislae and find several regions of strong homology among which is a potential ATP-bindlng site homology shared by a variety of ATP-binding proteins including protein kinases encoded by mammalian oncogenes