Complete Genome Sequence of Salmonella enterica Serovar

Salmonella is one of the major pathogenic bacteria that cause food poisoning. To elucidate the host infectionmechanism of Sal-monella enterica serovar Typhimurium-targeting phages, the bacteriophage SPN3UB was isolated from a chicken fecal sample. This phage belongs morphologically to the Siphoviridae family and infects the host via the O antigen of lipopolysaccharide (LPS). To further understand its infectionmechanism, we completely sequenced and analyzed the genome. Here, we announce its complete genome sequence and report major findings from the genomic analysis results. Salmonella is a pathogenic bacterium that causes salmonellosisvia contaminated foods (6, 7). To develop phages as a biocon-trol agent for controlling this pathogen in foods, understanding of the host infection mechanism of Salmonella phages is important (2, 3, 10). While the O antigen of lipopolysaccharides (LPS) is a common host receptor for infection in the Myoviridae (FelixO1) (11) and Podoviridae (P22, 34) families (4, 13, 14), phages in the Siphoviridae family that use this host receptor, such as SETP3, are rare (8). The S. Typhimurium-targeting SPN3UB phage, which belongs to the Siphoviridae family, could not infect the rfaL (O-antigen ligase)-deficientmutant strain of S.TyphimuriumSL1344 (data not shown), suggesting that it infects the host via the O antigen of LPS (12). To further understand its host infection mechanism, we completely sequenced and analyzed the genome. Phage genomic DNA was isolated using the standard alkaline lysis method (15) and was sequenced using a Genome Sequencer FLX Titanium instrument by Macrogen (Korea). Assembly of quality filtered reads was performed using a 454 Newbler 2.3 as-sembler, and open reading frames (ORFs) were predicted usin