IN VITRO PROPAGATION OF CLEMATIS X JACKMANII

This paper reports in vitro clonal propagation of Clematis x jackmanii. Explant material was axillary buds from one year old branches collected from mature plants in the field in March or July. Three basal media were compared: MS (Murashige and Skoog, 1962, LF (Lee Fossard), 1972, QL (Quoirin and Lepoivre, 1977) containing GA3 (0.1g l-1) and IBA (0.01g l-1) during initiation and GA3 (0.1mg l-1), BA (1.0mg l-1) and NAA (0.2mg l-1) during multiplication, (2, 3, 4). The regeneration ability of axillary buds explants ( % of regenerated explants) was recorded 30 days after the culture initiation. The multiplications rate (MR) as average number of plantlets/original explant was recorded for 5 subcultures and the rooting ability (percentage of rooted plants) was recorded after 30 days, (1). The multiplication rate depended on basal medium, variety and number of subcultures. The best multiplication medium was QL resulting in 10.5 shoots/explant. The multiplication rate on MS and LF medium was similar. The best result regarding in vitro regeneration was recorded for grown on QL medium (83.3%). The best time of the year to collect explant material was March, at the beginning of growing season. The highest multiplication rate 10.5 plants/explant after 5 subcultures was on QL medium. Shoots were rooted on QL medium containing either IBA (0.250mg l-1) or NAA (0.250mg l-1)