Discrimination of the toxigenic dinoflagellates Alexandrium tamarense and A. ostenfeldii in co-occurring natural populations from Scottish coastal waters

Blooms of the toxic dinoflagellate Alexandrium tamarense (Lebour) Balech, a known producer of potent neurotoxins associated with paralytic shellfish poisoning (PSP), are common annual events along the Scottish east coast. The co-occurrence of a second Alexandrium species, A. ostenfeldii (Paulsen) Balech & Tangen is reported in this study from waters of the Scottish east coast. The latter species has been suspected to be an alternative source of PSP toxins in northern Europe. Recent identification of toxic macrocyclic imines known as spirolides in A. ostenfeldii indicates a potential new challenge for monitoring toxic Alexandrium species and their respective toxins in natural populations. In mixed phytoplankton assemblages, Alexandrium species are dicult to discriminate accurately by conventional light microscopy. Species-specific rRNA probes based upon 18S and 28S ribosomal DNA sequences were developed for A. ostenfeldii and tested by dot-blot and fluorescence in situ hybridization (FISH) techniques. Hybridization patterns of A. ostenfeldii probes for cultured Alexandrium isolates, and cells from field populations from the Scottish east coast, were compared with those of rDNA probes for A. tamarense and a universal dinoflagellate probe. Alexandrium cell numbers in field samples determined by whole-cell in situ hybridization were much lower than those determined by optical microscopy with the Utermöhl method involving sedimentation chambers, but the results were highly correlated (e.g. r2=0.94; n=6 for A. tamarense). Determination of spirolides and PSP toxins by instrumental analysis on board ship demonstrated the presence of both toxi