Creatine kinase and phosphorylase in cardiac lymph: coronary occlusion and reperfusion

Creatine kinase and phosphor&se in cardiac lymph: coronary occlusion and reperfusion. Am. J. Physiol. 248 (Heart Circ. Physiol. 17): H350-H359, 1985.-Cardiac lymph, collected from conscious dogs, was monitored for glycogen phosphorylase and creatine kinase (CK) enzymatic activity during control state, circumflex coronary artery (CFX) occlusion, and reper-fusion. CFX occlusions, lasting for intervals as short as 10 min, initiated a release of phosphorylase and CK into the cardiac lymph, which was immediately observed during reperfusion of the ischemic tissue. Blood plasma levels did not appear for several hours. In the absence of reperfusion, the appearance of enzymes in cardiac lymph was delayed and peaked later. Gly-cogen phosphorylase and CK entered the lymph in greater quantities with reperfusion as the length of occlusion was increased. Histological examination of multiple sections of th