ReseaRch aRticLe Essential Gene Profiles in Breast, Pancreatic

  • Ovarian Cancer Cells
  • Richard Marcotte
  • Kevin R. Brown
  • O Suarez
  • Azin Sayad
  • Konstantina Karamboulas
  • Paul M. Krzyzanowski
  • Fabrice Sircoulomb
  • Mauricio Medrano
  • Yaroslav Fedyshyn
  • Judice L. Y. Koh
  • Dewald Van Dyk
  • Bohdana Fedyshyn
  • Marianna Luhova
  • Glauber C. Brito
  • Franco J. Vizeacoumar
  • Frederick S. Vizeacoumar
  • Ro Datti
  • Dahlia Kasimer
  • Alla Buzina
  • Jeffrey L. Wrana
  • Robert Rottapel
  • Benjamin G. Neel
  • Jason Moffat
Publication date
September 2016

Abstract

(“synthetic lethality”)  can arise as a consequence of the genetic abnormalities in cancer cells,  as illustrated by the sensitivity of BRCA1/2-mutant  breast  cancer  cells  to  PARP  inhibitors (7,  8). The systematic  identification of  such synthetic  lethal relationships might suggest new drug targets (9). Comparison of the genetic abnormalities and functional  vulnerabilities of cancer cells should help researchers identify new drivers and provide insight into the complex systems biology of cancer. RNA interference  technology  has  enabled  genome-wide loss-of-function screens  in mammalian  cells. Most  screens have used  siRNAs,  usually  arrayed  in  multiwell  plates. Arrayed screens have focused mainly on specific gene fami-lies...

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