Original Research Article Demonstration of Multivariate Data Analysis for the Development of nanoemulsions containing Active Herbal Principle of Boswellia serratta for Topical Application

A b s t r a c t The chemometric techniques have been used to demonstrate the role of nanoemulsion components on release of active herbal principle present in the spray dried Boswellia serratta extract. Isopropyl myristate, Tween 80 and Transcutol PR were selectedafter determining the solubility of boswellic acids (BAs) and used to draw phase diagrams. Simplex lattice mixture design was applied to optimize the percentual composition of nanoemulsions.The Permeability coefficient and droplet size were modelled with set of variables by partial least squares (PLS). Partial least square regression analysis was done through Excel STAT to influence mixture composition on permeation behavior of drug from the nanoemulsion as calculated models revealed good predictive abilities.Ex vivo skin permeation and in vivo anti-inflammatory study were conducted to evaluate the potential of optimized nanoformulations. About 3.25fold increase in flux was seen in case of nanoemulsion, nanogel showed 1.45 fold increase in flux as compared to carbopol gel with highest enhancement ratio 4.57 and 1.59 respectively. Physicochemically stable and non-irritant hydrogel showed significant percentage inhibition in rat paw edema. Our study illustrated scientific and statistical evidencefor the potential of developed nanoemulsion as possible alternative to traditional topical formulations