Three different turkey luteinizing hormone receptor (tLH-R) isoforms I: characterization of alternatively spliced tLH-R isoforms and their regulated expression in diverse tissues. Biol Reprod 2000

Using combinations of reverse transcription-polymerase chain reaction (RT-PCR) and 59- and 39-rapid amplification of cDNA ends, three different, alternatively spliced, partial turkey LH re-ceptor (tLH-R) cDNA isoforms were characterized from ovarian mRNA. The first cDNA (tLH-Rintact) showed 98 % and 72–75% similarity with chicken and mammalian LH-R sequences, re-spectively. The second cloned cDNA isoform (tLH-Rinsert) con-tained an in-frame TGA stop codon within an 86-base pair in-sertion that was located in the extracellular domain of the sev-en-transmembrane region. The tLH-Rinsert isoform could encode a truncated soluble protein isoform that lacked the transmem-brane region. The third cDNA isoform truncated the transmem-brane region (tLH-Rtrunc) and was derived by the deletion of the last exon by incomplete splicing. Generation of multiple tran