Evaluating the Number of Stages in Development of Squamous Cell and Adenocarcinomas across Cancer Sites Using Human Population-Based Cancer Modeling

Background: Adenocarcinomas (ACs) and squamous cell carcinomas (SCCs) differ by clinical and molecular characteristics. We evaluated the characteristics of carcinogenesis by modeling the age patterns of incidence rates of ACs and SCCs of various organs to test whether these characteristics differed between cancer subtypes. Methodology/Principal Findings: Histotype-specific incidence rates of 14 ACs and 12 SCCs from the SEER Registry (1973– 2003) were analyzed by fitting several biologically motivated models to observed age patterns. A frailty model with the Weibull baseline was applied to each age pattern to provide the best fit for the majority of cancers. For each cancer, model parameters describing the underlying mechanisms of carcinogenesis including the number of stages occurring during an individual’s life and leading to cancer (m-stages) were estimated. For sensitivity analysis, the age-period-cohort model was incorporated into the carcinogenesis model to test the stability of the estimates. For the majority of studied cancers, the numbers of m-stages were similar within each group (i.e., AC and SCC). When cancers of the same organs were compared (i.e., lung, esophagus, and cervix uteri), the number of m-stages were more strongly associated with the AC/SCC subtype than with the organ: 9.7960.09, 9.9360.19 and 8.8060.10 for lung, esophagus, and cervical ACs, compared to 11.4160.10, 12.8660.34 and 12.0160.51 for SCCs of the respective organs (p,0.05 between subtypes). Most SCCs had more than tenm-stages while ACs had fewer than ten m-stages. The sensitivity analyses of the model parameters demonstrated the stabilit