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Burkholderia terrae BS001 is a soil bacterium which was originally isolated from the mycosphere of the ectomycorrhizal fungus Laccaria proxima. It exhibits a range of fungus-interacting traits which reveal its propensity to actively interact at fungal interfaces. Here, we present the approximately 11.5-Mb (GC content, 61.52%) draft genome sequence of B. terrae BS001 with the aim of providing insight into the genomic basis of its ecological success in fungus-affected soil settings. Burkholderia terrae strain BS001 is a Gram-negative, rod-shaped member of the Betaproteobacteria which was isolated from acidic sandy soil underneath the foot of Laccaria proxima fruiting bodies (8). Similar Burkholderia terrae types were also positively selected in soil by the growing fungus Lyophyllum sp. strain Karsten (4, 9). Remarkably, B. terrae BS001 migrates with growing fungal hyphae and assists other bacteria, e.g., Dyella ja-ponica strain BS003, to comigrate (7). Interestingly, the group of so-called beneficial Burkholderia species (6) also contains the endofungal Burkholderia rhizoxi-nica (5). The related B. terrae BS001 is also fungus interacting