Original Communications Alanine-Glutamine Dipeptide (AGD) Inhibits Expression of Inflammation-Related Genes in Hemorrhagic Shock

ABSTRACT. Background: Inflammatory factors play an important role in the production of cellular damage after shock and reperfusion. Glutamine has been used to modulate the inflammatory response. Alanine-glutamine dipeptide (AGD) is a glutamine source. The hypothesis of the present study is that AGD given during resuscitation will suppress postshock expression of messenger ribonucleic acid (mRNA) for tumor necrosis factor (TNF-), interleukin-1 (IL-1) and inducible nitric oxide synthase (iNOS). Methods: Male Sprague-Dawley rats (n 74, 350 g 30 g) were randomly assigned to 5 groups. Under isoflurane anesthesia, the fem-oral artery and vein were cannulated. Hemorrhagic shock was induced by withdrawing blood through the arterial can-nula until the mean arterial pressure (MAP) was 25–30 mm Hg and maintained at the level for 30 minutes with furthe