A Radiolabeled Antiglobulin Test for Crossmatching Platelet Transfusions

Despite the use of HIA-matched platelets for alloimmu-nized recipients. transfusion failures occur. In order to reduce these failures, we investigated the use of a radiola-beled antiglobulin technique for platelet crossmatching. The principle of the test is that of an indirect Coombs test using 1251 labeled goat anti-human lgG. Incompatibility is determined by calculating a radioactivity antiglobulin test (RAGT) index. Using this technique. we performed 89 crossmatches on 1 9 leukemic or aplastic patients who were refractory to random donor platelets and receiving varying degrees of HIA-matched platelets. Effectiveness of the transfusion was assessed from the posttransfusion corrected platelet count increment (CCI) determined at 1 T H ROM BOCYTOPEN IC PATIENTS receiving multiple transfusion can often become alloimmu