volume 11 Number 15 1983 Nucleic Acids Research Complete nudeotide sequence of the Escherichia coli gdhA gene

The DNA sequence of the gdhA gene of E s c h e r i c h i a c o l i K12, which encodes the 447 amino acid polypeptide subunit of NADP-speciflc glutamate dehydrogenase, is presented. The deduced protein sequence is strongly homologous to the corresponding enzyme of the eukaryotic fungus Neurospora crasBa. The upstream DNA sequence includes several overlapping promoter consensus sequences. The downstream DNA sequence contains inverted repeats, predicted as forming long stable stem-loop structures in RNA, homologous to those found in several enterobacterial intergenic regions