Biphasic decay of latently infected CD4+ T cells in acute human immunodeficiency virus type 1 infection

Latent infection of resting CD4+ T cells represents a major barrier to eradication of human immunode®ciency virus type 1 (HIV-1). The establishment and rate of decay of latent HIV-1 in resting CD+ T cells from 9 acute seroconverters, 7 of whom began to receive highly active antiretroviral therapy (HAART) shortly after presentation, were studied. Before the initiation of therapy, these patients had very high frequencies of latently infected CD4+ T cells, with a median frequency of 205 infectious units per million resting CD4+ T cells. These values are>1 log higher than those seen in chronically infected patients who are not undergoing HAART. The number of latently infected cells declined dramatically after initiation of HAART but then tended to level off at a low but stable level. The biphasic decay of latent HIV in resting CD4+ T cells in acute seroconverters supports current models of pre- and postintegration latency. Latently infected CD41 T cells are an important reservoir for individuals infected with human immunode®ciency virus type 1 (HIV-1) [1±6]. Latently infected cells are thought to arise when productively infected CD41 T lymphoblasts escape both immune effector mechanisms and the cytopathic effects of th