SPECTROPHOTOMETRIC DETERMINATION OF AMPICILLIN AND CLOXACILLIN IN PURE AND FIXED DOSAGE FORMS THROUGH CHARGE TRANSFER COMPLEXATION

Simple, precise and accurate visible spectrophotometric procedure was developed for the quantitative determination of two antibacterials, ampicillin and cloxacillin in a fixed dose combination. The method exploits the formation of charge transfer complexes between the antibacterials and 2,3-dichloro-5,6-dicyano-1,4-benzoquinone (DDQ) to determine the components of the drugs in a dosage form. The effect of different variables on the development and stability of the complexes were studied and optimized. Beer’s law was obeyed. The method was successfully applied to the determination of the target analytes in different brands of the fixed dose combination