DW: Mapping of monoclonal antibodies to human factor IX

We used recombinant DNA techniques to map a panel of six monoclonal antibodies (MoAbs) to regions of the human factor IX molecule. A-2 maps to 1 7 amino acids at the amino terminus of the heavy chain of IXa; 2D5. an inhibitor of clotting. is defined to 36 amino acids of the first EGF-like domain of human factor IX. A-4. A-5. ClOD, and FXCOO8 all F UNCTIONAL FACTOR IX is essential for normal hemostasis. A defective factor IX molecule results in hemophilia B, an X-linked hereditary bleeding disorder. Human factor IX has a molecular weight (mol wt) of-57,000 daltons and contains ‘-.17 % carbohydrate.”2 The first 12 amino terminal glutamic acid residues of the zymogen are modified to ‘y-carboxyglutamic acids (Gla) in a posttranslational vitamin K-dependent reaction.2’5 These Gla residues are required for function and are responsible for most of the calcium-dependent binding of factor IX t