Purification of Myeloperoxidase from

Increases of plasma concentra-tions of neutrophil myeloperoxidase (MPO) can be used as markers of polymorphonuclear leucocytes (PMN) activation in pathological situations (sepsis, acute lung injury, acute inflammation). To develop an assay for measurement of plasma MPO in horses during the above-mentioned infectious and inflamma-tory conditions, MPO was purified from equine PMN isolated from blood anticoagulated with citrate. PMN were extracted in a salin