American Journal of Advanced Drug Delivery www.ajadd.co.uk American Journal of Advanced Drug Delivery www.ajadd.co.uk Original Article Method Development and Validation of Stability Indicating RP-HPLC Method for Simultaneous Estimat

A new rapid, precise and sensitive reverse phase high performance liquid chromatographic (RP-HPLC) method has been developed and validated for the estimation of Perindopril and Amlodipine simultaneously in combined dosage form. The two components Perindopril and Amlodipine were well resolved on an isocratic method, C18 column, utilizing a mobile phase composition of acetonitrile: methanol: a mixed buffer of 0.02M Potassium dihydrogen phosphate buffer and 0.02M Sodium dihydrogen Phosphate buffer with 1mL Tri ethyl amine (40:20:40), v/v, pH 5.0) at a flow rate of 1.0 mL/min with UV detection at 226 nm. The retention time of Perindopril and Amlodipine were 2.9 min and 4.9 min respectively. The developed method was validated for specificity, linearity, precision, accuracy, limit of detection (LOD), limit of quantification (LOQ) and robustness as per ICH guidelines