A rapid method for chromatin structure analysis in the filamentous fungus Aspergillus nidulans. Nucleic Acids Res 25: 3955–3956

A rapid method for nuclease digestion of Aspergillus nidulans chromatin is described. It overcomes the need for nuclear purification or protoplast preparation. The method is valid for the analysis of the nucleosomal repeat length in bulk chromatin, and allows the analysis of nucleosome phasing at a specific locus. Eukaryotic nuclear DNA is packaged in nucleosomes and higher-order chromatin structures. This organisation restricts the accessibility of DNA to proteins, including RNA polymerases and transcription factors. Transcriptional activation is frequently accompanied by alterations in the chromatin structure of regulatory sequences. These alterations can usually be detected as DNase I hypersensitive sites (DHSs) (1). Furthermore, nucleosomes can be quite precisely positioned with respect to specific DN