Comparison of corticotropin-releasing factor and acetylcholine on catecholamine secretion in dogs

releasing factor and aeetylcholine on catecholamine secretion in dogs. Am. J. Physiol. 253 (Regulatory Integrative Comp. Phys-iol. 22): R209-R215, 1987.-To test whether corticotropin-releasing factor (CRF) is a secretagogue for adrenal secretion of catecholamines, a preparation was developed that permits measurement of adrenal venous output in response to in vivo arterial injection into the dog adrenal gland. Dogs were pre-pared with catheters in the lumboadrenal vein for monitoring adrenal blood flow and secretion rate of epinephrine and nor-epinephrine and in the lumboadrenal and inferior phrenic arteries for adrenal injection. Under pentobarbital anesthesia 48 h after surgery, dogs received a series of intra-adrenal injections that included acetylcholine (0.2-200 nmol), CRF (2.0-20.0 nmol), and diluent. There was a log dose-response relationship before epinephrine secretion and norepinephrin