Zahedan Journal of Research in Medical Sciences Journal homepage: www.zjrms.ir Antibacterial Activity of Propolis Ethanol Extract against Antibiotic Resistance Bacteria Isolated from Burn Wound Infections

Background: Burn wound is a suitable site for incidence of resistant infections; thus, the research for finding effective drugs against this infection is necessary. The purpose of this study was to determine antibacterial activity of Isfahan bee propolis extracts against beta-lactamase producing bacteria isolated from burn wound infections. Materials and Methods: Ethanol extract of Isfahan bee propolis was prepared by 28 g of propolis in 100 ml of 70 % ethanol. Antibacterial activity of ethanol extracts were evaluated against beta-lactamase producing bacteria (Staphylococcus aureus and Pseudomonas aeruginosa) isolated of burn wound infection by well diffusion method. Broth serial dilution method was used to determine MBC of extract. Beta-lactamase production of isolates was detected by iodometric test, imipenem-EDTA combined disk test and imipenem-EDTA double-disk synergy test. Results: Ethanol extract of propolis was found to be the most effective against S. aureus strains (inhibition zone=17.66±0.47 mm) than P. aeruginosa strains (inhibition zone=7 mm). The MIC and MBC values of the extracts against S. aureus strains were 0.0143 and 0.0286 mg/ml and these values against P. aeruginosa strains were 0.75 and 1.5 mg/ml