Journal of Controlled Release Original Article γ-Polyglutamic acid-coated vectors for effective and safe gene therapy

In the present study, we developed some novel gene delivery vectors, coated cationic complexes with γ-polyglutamic acid (γ-PGA) for effective and safe gene therapy. Cationic complexes were constructed with pDNA and cationic vectors, such as poly-L-arginine hydrochloride (PLA), poly-L-lysine hydrobromide (PLL), N-[1-(2, 3-dioleyloxy) propyl]-N, N, N-trimethylammonium chloride (DOTMA)-cholesterol (Chol) liposomes, and DOTMA-dioleylphosphatidylethanolamine (DOPE) liposomes. The cationic complexes showed high gene expression with strong cytotoxicity in melanoma B16-F10 cells. The cationic complexes were also strongly toxic to erythrocytes. On the other hand, the γ-PGA was able to coat all cationic complexes and form stable nano-sized particles with negative charges. These γ-PGA-coated complexes had high gene expression without cytotoxicity an