Article Enzymatic Hydrolysis of N-protected 2-Hydroxymethylpiperidine Acetates

Abstract. Liver acetone powders (LAPs) were used as hydrolase sources to biocatalyze the hydrolysis of N-protected-2-hydroxymeth-ylpiperidine acetates (N-Ts and N-Boc). Different reaction conditions were evaluated, like source of LAP (cat, guinea pig, chicken, dog, sheep, bovine, rat, mouse, pig and rabbit), proportion of cosolvent, type of cosolvent and pH. Key words: Liver acetone powder, 2-hydroxymethylpiperidine, bio-catalysis, hydrolysis. Resumen. Como fuente de hidrolasas se usaron polvos acetónicos de hígado (PAH), para biocatalizar la hidrólisis de los acetatos de la 2-hidroximetilpiperidina N-protegida (N-Ts y N-Boc). Se evaluaron diferentes condiciones de reacción, como la fuente del PAH (gato, cuyo, pollo, perro, borrego, res, rata, ratón, cerdo y conejo), la pro-porción de codisolvente, tipo de codisolvente y pH