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Approved for public relea-se; distribution unlimited 1 ".-ABSTRACT (Maximum 200 words) oThe purpose of this grant is to investigate the metabolic and physiological factors M " G determining the extent of ischemic damage in a model of brief repetitive ischemia. _ The model is intended to simulate the effects of Gz induced blackout experienced by fighter pilots undergoing high gravitational stress maneuvers. To achieve this goal a rat model was developed whereby ischemia could be induced remotely (under computer Sregulation) by inflation of occlusive cuff about the common carotid artery of the •OO rat. Metabolic parameters were determined by performing in vivo NMR measurements. • • throughout the repetitive ischemic insult. The non-destructive nature of the N-MR W0 measurement permits complete time courses to be monitored in each animal thereby minimizing animal variability. NMR measurements of metabolite content are then used in conjunction with histologic evaluations of neuronal death to assess which metabolic factors have the strongest influence on ischemic damage. During the pas