Integrated capacitive biosensors based on filamentous phages

References • In this work, we report on the succesfull selection and identification of peptide motives that exhibit a specific affinity to anodic alumina surfaces when multivalently displayed on a filamentous phage. • It was also demonstrated that, for a selected phage clone, a chemical functionalisation (biotinylation) of the bacteriophage does not deteriorate its specific affinity to anodic alumina. Such biotinylated bacteriophages, after being immobilised onto an alumina surface, also allow for the quantitative detection of streptavidine using an ELISA protocol. • When immobilising selected filamentous phages on interdigitated Al/Al2 O3 electrodes, a characteristic change of the capacitance signal with frequency is observed. • These results are believed to pave the way for shifting the surface design of integrated biosensing devices from traditional, chemically modified synthetic surfaces towards molecular linkers based on genetically engineered polypeptides. Substrates and phage