Retinoid-Induced Transformed Mouse

blaltl (Balb/3T12-3 celli) were readily detached from the dish lurface In an EDTA-medlated detachment allay. Retlnolc acld-treated celli dllplayed Increaled adhesion to the culture dllh lurface. The eHect of retlnolc acid on the adhesion of Balb/3T12-3 celli wal dOle-dependent In the range of 0.05-5 ~g/ml (0.17-17 ~M) In an al..y performed on celli cultured for 3 dayl In the prelence of the retinoid. The earllelt eHeet on adhellon wal deteeted at 2 dayl of culture In the prelence of 17 ~M retlnolc acid. The Increale In adhellon displayed by retlnolc acid-treated celli wal rapidly 10lt upon r~moval of the retinoid from the culture medium. Synthetic retlnoldl were telted for their eetlvlty In Inducing adhellon of cultured Balb/3T12-3 celli. Retinol, retlnolc acid, and their 5,6-epoxy derlvatlvel were the mOlt active, Ihowlng activity at 1 ~g/ml. 13-c/a-Retlnolc eeld and the dlmethyl8cetylcyclopentenyl and trlmethylmethoxyphenyl derlva