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ii Mammalian transient receptor potential (TRP) family is composed of 28 members and they are extensively expressed in many tissues and play important roles in cell death, calcium absorption, and sensation of temperature, pain, osmolarity, and hearing, etc. There are at least six thermo-sensitive TRPs: TRPA1, TRPM8, TRPV1-4. TRPV3 is expressed in a variety of tissues including brain, spinal cord, dorsal root ganglia (DRG), skin, heart, testis, etc. TRPV3 is activated at temperature>33 ºC and may be important for the sensation of warmth. The goal of this study is to understand the activation and regulation of TRPV3 and some other TRP channels. The data indicate that TRPV3 has many common properties shared with certain other TRP channels in activation and regulation. 2-amino epythoxydiphenyl borate (2APB) is a compound widely used to inhibit inositol (1, 4, 5) trisphosphate receptors (IP3Rs) and store-operated calcium entry. In this study, experiments including calcium measurements and patch clamp recordings indicate that 2APB is a common agonist for three TRPV channels: TRPV1,-V2 and –V3