Complete Genome of a Novel Endornavirus Assembled from Next- Generation Sequence Data

Endornaviruses have large double-stranded RNA (dsRNA) genomes that carry a single open reading frame (ORF). Here we re-port the complete genome of a novel endornavirus, assembled from next-generation sequence data generated from Vitis vinifer-a-extracted dsRNA. Two different fungal hosts have been identified for this virus, suggesting that horizontal transmission of the virus is possible. The family Endornaviridae is a new virus family (1) that con-tains viruses infecting plants, fungi, and oomycetes. Endorna-viruses have double-stranded RNA (dsRNA) genomes which carry a single open reading frame (ORF) (2). An RNA-dependent RNA polymerase (RdRp) domain is the only conserved domain among allmembers, while other domains, likemethyl-transferase, helicase, and glycosyltransferase, are present in some, but not all members (5). Endornaviruses are not encapsidated, they cause persistent infections, and horizontal transmissions are believed not to occur in plants or fungi (2, 5). Here we report the complete genome of a novel dsRNA virus, assembled fromnext-generation sequence data of dsRNApurifie