80 Clin Pathol 1993;46:840-845 Use of recombinant human parvovirus B 1 9 antigens in serological assays

Aims-To compare the sensitivity, speci-ficity, and practicality of recombinant proteins in serological tests for the detec-tion of human parvovirus B19 IgG and IgM. Methods-Indirect enzyme linked immunosorbent assays using B19 struc-tural proteins expressed in Escherichia coli were developed for the detection of B19 specific IgG and IgM (rELISA-G and rELISA-M). Cells infected with baculo-virus expressing B19 structural proteins were also used in an indirect immuno-fluorescence assay for IgG and Ig